logo

Bangladesh Agricultural University (BAU)

Publish Date: 16 Dec 2020

Isolation of peste des petits ruminants virus using primary goat kidney cell culture from kidneys obtained at slaughter

Dr. Mohammed Nooruzzaman

Share this on

Citation: Begum, S., Nooruzzaman, M., Hasnat, A., Parvin, M.M., Parvin, R., Islam, M.R., Chowdhury, E.H. (2020): Isolation of peste des petits ruminants virus using primary goat kidney cell culture from kidneys obtained at slaughter. Veterinary Medicine and Science, 10.1002/vms3.413. Advance online publication. https://doi.org/10.1002/vms3.413

Abstract
Background: Traditionally isolation of peste des petits ruminant virus (PPRV) is performed
in Vero cells that takes several blind passages before observing typical cytopathic
effects (CPEs). As an alternate, researchers have been using lamb kidney (LK)
cells but day-old lambs are difficult to obtain and requires animal sacrifice.
Objective: We established a primary goat kidney (GK) cell culture from the kidneys
obtained at slaughter.
Methods: The kidney of Black Bengal goats were collected from slaughter house and
processed to make single cell suspension. The cells were resuspended in appropriate
culture medium and maintained under optimum culture condition.
Results: The 80% confluent monolayer of GK cells was obtained after 15–20 days
post seeding. Upon infection with a field isolate of PPRV, the well-developed CPEs
characterized by cell rounding, vacuolation in the cytoplasm and fusion of cells were
observed after 48 hr post infection. Virus quantification in the culture supernatant
revealed more viral RNA in GK cells than LK cells. The multicycle growth analysis
of PPRV showed a steady increase in the virus loads in the culture supernatant of
infected GK cells, suggesting an adaptation of the PPRV in GK cells.
Conclusions: The findings suggest that primary GK cells can be successfully prepared
from the mature kidney cortical tissues and can be used for the isolation of PPRV.
This system could reduce the unnecessary sacrifice of lambs or kids. Since kidneys of
slaughtered goats are available throughout the year, using this protocol primary cell
culture from mature goat kidney can provide primary cells to the laboratory throughout
the year.
K E Y W O R D S
Black Bengal goats, goat kidney cell culture, peste des petits ruminants, virus isolation

Other Research